* 임상 시료 등에서 알코올과 산에 내성이 있는 박테리아 염색을 위한 키트입니다.

Kinyoun color kit [MB-80380]

Complete kit for coloring alcohol-acid resistant bacteria in clinical samples or culture media.

PACKAGE

PROCEDURE

1. Preparation and fixing

Use clean plates. Swab the culture or pathological material. Dry in the air and fix by passing rapidly over the flame. Other fixing methods can be used.

2. Staining

(* Before use the Gabett Solution, please heat in a water bath to 50 ~ 60°C until disappear the precipitation.)

During all phases of the above methods, the preparations must be washed in water free of alcohol-acid resistant species.

TAN-THIAM-HOK METHOD

· Cover the plate with Kinyoun Solution and leave in contact for 3 minutes.

· Wash with water for 30 seconds.

· Cover the plate with Gabett Solution and leave in contact for 1 minute.

·Wash with water and dry.

· Observe the preparation under the microscope using the immersion lens.

HOT DEVULDER METHOD

· Cover the plate with Kinyoun Solution and heat for 10 minutes.

· Wash with water.

· Cover the plate with Gabett Solution. Leave in contact for 5 minutes.

· Wash with water and dry.

· Observe the preparation under the microscope using the immersion lens.

COLD DEVULDER METHOD

· Immerse the plate in the Kinyoun Solution for about 3 hours.

· Wash with water.

· Cover the plate with Gabett Solution. Leave in contact for 5 minutes.

· Wash with water and dry.

· Observe the preparation under the microscope using the immersion lens.

STORE

Store Kinyoun Color Kit at 10-25°C in its original packaging. Do not store it near sources of heat and do not expose it to excessive temperature variations. If properly stored, the Kinyoun Color Kit (Tan-Thiam-Hok and Devulder method) can be used until the expiry date shown on the label. Do not use after this date. Dispose of it if there are signs of deterioration (solutions change color or there are signs of large precipitates).