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Rogosa Broth [MB-R1177]
Selective basal medium for lactobacilli isolation and enumeration.
PACKAGE
Description |
Quantity |
Cat. No |
Rogosa Broth |
500g |
MB-R1177 |
Rogosa Broth |
*500ml x 10 |
MB-R1177M |
Tween 80 supplement (*Çʼö÷°¡) |
50ml (½Ç¿Â) |
MB-T1861 |
Glacial Acetic Acid supplement (*Çʼö÷°¡) |
15ml (½Ç¿Â) |
MB-G0743 |
*500ml x
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PROCEDURE
Suspend 58.72 G of powder in 1 L of distilled or
deionized water. Add 1 mL of Tween 80 supplement (MB-T1861) and 1.32 mL of
Glacial Acetic Acid supplement (MB-G0743). Heat until completely dissolved. Boil for 2-3 minutes. Cool to 45-50¡ÆC. Dispense in sterile final tubes. Do not sterilize in autoclave and do not overheat.
STORE
The powder is very hygroscopic. Store the powder at room
temperature, in a dry environment, in its original container tightly closed and
use it before the expiry date on the label or until signs of deterioration or
contamination are evident. Store prepared medium at 2 - 8¡ÆC.
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* This product may have contamination risk during the manufacturing or delivering, so please check before use it.