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Terrific Broth [MB-T1556]
Medium used for the cultivation recombinant
strains of Escherichia coli.
PACKAGE
Description |
Quantity |
Cat. No |
Terrific Broth |
500g |
MB-T1556 |
Terrific Broth |
5kg |
MB-T1556-5kg |
Glycerol supplement (Çʼö ÷°¡) |
50ml (½Ç¿Â) |
MB-G1821 |
PROCEDURE
Suspend 47.6 G of powder in 1 L of
distilled or deionized water. Add 4.0 mL of Glycerol supplement (MB-G1821). Heat
to boiling until completely dissolved. Sterilize by autoclave at 121¡ÆC for 15
minutes. Cool to 45-50¡ÆC in water bath. Mix well. Dispense in tubes.
STORE
The powder is very hygroscopic. Store the
powder at room temperature, in a dry environment, in its original container
tightly closed and use it before the expiry date on the label or until signs of
deterioration or contamination are evident. Store prepared medium at 2 - 8¡ÆC.