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M9 Minimal Broth [MB-M1191]
Medium used for cultivation and enumeration
of E.coli for molecular biology
purpose.
PACKAGE
Description |
Quantity |
Cat. No |
M9 Minimal Broth |
500g |
MB-M1191 |
M9 Minimal Broth |
500ml x 10 |
MB-M1191M |
* M9 Minimal Broth supplement |
1 vial/ 10ml (³Ãµ¿) |
MB-M0732 |
* 500ml x 10 Á¦Ç°ÀÇ °æ¿ì, 500ml ¹èÁö¸¦ Á¦Á¶ÇÒ ¼ö ÀÖ´Â ÆÄ¿ì´õ ¾ç¸¸Å ¼ÒºÐ µÇ¾îÀÖ´Â Á¦Ç°ÀÔ´Ï´Ù.
* M9 Minimal Broth supplement (º°µµ ±¸¸Å ÇÊ¿ä)
Contents |
g |
1 vial contents (each vial is sufficient for 500mL of
medium) |
|
Glucose |
2.0g |
Thiamine Hydrochloride |
0.0005g |
Magnesium Sulfate |
0.123g |
Calcium Chloride |
0.00735g |
PROCEDURE
Suspend 10.5 G of powder in 1 L of distilled or deionized water. Heat to
boiling until completely dissolved. Sterilize by autoclave at 121¡ÆC for 15
minutes. Cool to 45 - 50¡ÆC in water bath. Aseptically add 2 vials of M9 Minimal
Broth supplement (MB-M0732). Mix well. Pour into tubes.
STORE
The powder is very hygroscopic. Store the powder at room temperature, in a
dry environment, in its original container tightly closed and use it before the
expiry date on the label. Store prepared medium at 2 - 8¡ÆC.
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