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M9 Minimal Broth, MB-M1191
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      M9 Minimal Broth [MB-M1191]

       

       

       

      Medium used for cultivation and enumeration of E.coli for molecular biology purpose.

       

       

      PACKAGE

       

      Description

      Quantity

      Cat. No

      M9 Minimal Broth

      500g

      MB-M1191

      M9 Minimal Broth

      500ml x 10

      MB-M1191M

      * M9 Minimal Broth supplement

      1 vial/ 10ml (³Ãµ¿)

      MB-M0732

      * 500ml x 10 Á¦Ç°ÀÇ °æ¿ì, 500ml ¹èÁö¸¦ Á¦Á¶ÇÒ ¼ö Àִ ÆÄ¿ì´õ ¾ç¸¸Å­ ¼ÒºÐ µÇ¾îÀִ Á¦Ç°ÀÔ´Ï´Ù.



       

       

      * M9 Minimal Broth supplement (º°µµ ±¸¸Å ÇÊ¿ä)

       

      Contents

      g

      1 vial contents (each vial is sufficient for 500mL of medium)

      Glucose

      2.0g

      Thiamine Hydrochloride

      0.0005g

      Magnesium Sulfate

      0.123g

      Calcium Chloride

      0.00735g

       

       

       

       

       

       

      PROCEDURE

       

      Suspend 10.5 G of powder in 1 L of distilled or deionized water. Heat to boiling until completely dissolved. Sterilize by autoclave at 121¡ÆC for 15 minutes. Cool to 45 - 50¡ÆC in water bath. Aseptically add 2 vials of M9 Minimal Broth supplement (MB-M0732). Mix well. Pour into tubes.

       

       

      STORE

       

      The powder is very hygroscopic. Store the powder at room temperature, in a dry environment, in its original container tightly closed and use it before the expiry date on the label. Store prepared medium at 2 - 8¡ÆC.

       

       

       

       

       




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      M 9 Minimal Broth supplement, MB-M0732

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