* KCN ³»¼º¿¡ ±âÃÊÇÑ
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KCN Broth [MB-K0960]
Medium used for the identification and determination of Enterobacteriaceae on the basis of KCN
tolerance.
PACKAGE
Description |
Quantity |
Cat. No |
KCN Broth |
500g |
MB-K0960 |
KCN Supplement (*Çʼö÷°¡) |
1 vial / 5ml (³ÃÀå) |
MB-K3018 |
* KCN Supplement (º°µµ ±¸¸Å ÇÊ¿ä)
Contents |
g |
1 vial contents (each vial is sufficient for 500mL of
medium) |
|
KCN (Potassium Cyanide) |
0.0375 g |
PROCEDURE
Suspend 13.87 G powder in 1 L of distilled or deionized
water. If necessary, adjust pH to 7.6 ¡¾ 0.2. Heat to boiling until completely
dissolved. Sterilize by autoclave at 121¡ÆC for 15 minutes. Cool to 45 - 50¡ÆC in
water bath. Aseptically add 2 vials of KCN supplement (MB-K3018). Pour into
tubes. KCN is highly toxic, so always be careful when using KCN. Work must be
performed in a hood.
STORE
The powder is very hygroscopic. Store the powder at room
temperature, in a dry environment, in its original container tightly closed and
use it before the expiry date on the label or until signs of deterioration or
contamination are evident. Store prepared medium at 2 - 8¡ÆC.