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M9 Minimal Broth [MB-M1191]
Medium used for cultivation and enumeration
of E.coli for molecular biology purpose.
PACKAGE
Description |
Quantity |
Cat. No |
M9 Minimal Broth |
500g |
MB-M1191 |
M9 Minimal Broth |
500ml x 10 |
MB-M1191M |
* M9 Minimal Broth supplement |
1 vial/ 10ml (³Ãµ¿) |
MB-M0732 |
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* M9 Minimal Broth supplement (º°µµ ±¸¸Å ÇÊ¿ä)
Contents |
g |
1 vial contents (each vial is sufficient for 500mL of
medium) |
|
Glucose |
2.0g |
Thiamine Hydrochloride |
0.0005g |
Magnesium Sulfate |
0.123g |
Calcium Chloride |
0.00735g |
PROCEDURE
Suspend 10.5 G of powder in 1 L of distilled or deionized water. Heat to
boiling until completely dissolved. Sterilize by autoclave at 121¡ÆC for 15
minutes. Cool to 45-50¡ÆC in water bath. Aseptically add 2 vials of M9 Minimal
Broth supplement(MB-M0732). Mix well. Dispense in tubes.
STORE
The powder is very hygroscopic. Store the powder at room temperature, in a
dry environment, in its original container tightly closed and use it before the
expiry date on the label or until signs of deterioration or contamination are
evident. Store prepared medium at 2 - 8¡ÆC.
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