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F/2 Broth, MB-F0701
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      F/2 Broth [MB-F0701]

                                                                                                                                                         




       

      Medium used for the cultivation and enumeration of coastal marine algae, especially diatoms.

       

       

       

      PACKAGE

       

      Description

      Quantity

      Cat. No

      F/2 Broth

      500g

      MB-F0701

      F/2 Trace Metal Solution (*Çʼö÷°¡)

      1vial/ 0.5ml (³Ãµ¿)

      MB-F0766

      F/2 Vitamin Solution (*Çʼö÷°¡)

      1vial/ 0.25ml (³Ãµ¿)

      MB-F0767

       

       

      * F/2 Trace Metal Solution (º°µµ ±¸¸Å ÇÊ¿ä)

       

      Contents

      g

      1 vial contents (each vial is sufficient for 500mL of medium)

      Iron (III) Chloride Hexahydrate

      1.575 mg

      Ethylenediaminetetraacetic Acid Disodium Salt Dihydrate

      2.18 mg

      Copper (II) Sulfate Pentahydrate

      4.9 §¶

      Sodium Molybdate Dihydrate

      3.15 §¶

      Zinc Sulfate Heptahydrate

      11.0 §¶

      Cobalt (II) Chloride Hexahydrate

      5.0 §¶

      Manganese (II) Chloride Tetrahydrate

      90.0 §¶

       

       

      * F/2 Vitamin Solution (º°µµ ±¸¸Å ÇÊ¿ä)

       

      Contents

      §¶

      1 vial contents (each vial is sufficient for 500mL of medium)

      Thiamine HCl

      50.0 §¶

      Biotin

      0.25 §¶

      Cyanocobalamin

      0.25 §¶

       

       

       

      PROCEDURE

       

      Suspend 0.11 G of powder in 1 L of seawater. Heat to boiling until completely dissolved. Sterilize by

      autoclave at 121¡ÆC for 15 minutes. Cool to 45 - 50¡ÆC in water bath. Aseptically add 2 vials of F/2 Trace Metal Solution (MB-F0766) and 2 vials of F/2 Vitamin Solution (MB-F0767). Mix well. Pour into tubes.

       

       

      STORE

       

      The powder is very hygroscopic. Store the powder at room temperature, in a dry environment, in its original container tightly closed and use it before the expiry date on the label. Store prepared medium at 2 - 8¡ÆC.

       

       

       





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