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BL Broth [MB-B1602]
Medium used for the cultivation and enumeration of Bifidobacterium spp.
PACKAGE
Description |
Quantity |
Cat. No |
BL Broth |
500g |
MB-B1602 |
Sheep Blood Defibrinated (*ÅÃ1) |
50ml (³ÃÀå) |
MB-S1876 |
Horse Blood Defibrinated (*ÅÃ1) |
50ml (³ÃÀå) |
MB-H1883 |
*Sheep Blood
Defibrinated (MB-S1876) ¶Ç´Â Horse
Blood Defibrinated (MB-H1883) Áß ¼±ÅÃÇÏ¿© Çʼö ÷°¡
PROCEDURE
Suspend 45.23 G of powder in 1 L of distilled or
deionized water. Heat to boiling until completely dissolved. Sterilize by
autoclave at 121¡ÆC for 15 minutes. Cool to
45 - 50¡ÆC in water bath. Aseptically add 5% of Horse
Blood Defibrinated (MB-H1883) or Sheep Blood Defibrinated (MB-S1876). Mix well.
Pour into tubes.
STORE
The powder is very hygroscopic. Store the powder at room
temperature, in a dry environment, in its original container tightly closed and
use it before the expiry date on the label or until signs of deterioration or
contamination are evident. Store prepared medium at 2 - 8¡ÆC.