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M9 Minimal Broth, MB-M1191
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      M9 Minimal Broth [MB-M1191]

       

       

       

      Medium used for cultivation and enumeration of E.coli for molecular biology purpose.

       

       

      PACKAGE

       

      Description

      Quantity

      Cat. No

      M9 Minimal Broth

      500g

      MB-M1191

      M9 Minimal Broth

      500ml x 10

      MB-M1191M

      * M9 Minimal Broth supplement

      1 vial/ 10ml (³Ãµ¿)

      MB-M0732

      * 500ml x 10 Á¦Ç°ÀÇ °æ¿ì, 500ml ¹èÁö¸¦ Á¦Á¶ÇÒ ¼ö Àִ ÆÄ¿ì´õ ¾ç¸¸Å­ ¼ÒºÐ µÇ¾îÀִ Á¦Ç°ÀÔ´Ï´Ù.



       

       

      * M9 Minimal Broth supplement (º°µµ ±¸¸Å ÇÊ¿ä)

       

      Contents

      g

      1 vial contents (each vial is sufficient for 500mL of medium)

      Glucose

      2.0g

      Thiamine Hydrochloride

      0.0005g

      Magnesium Sulfate

      0.123g

      Calcium Chloride

      0.00735g

       

       

       

       

       

       

      PROCEDURE

       

      Suspend 10.5 G of powder in 1 L of distilled or deionized water. Heat to boiling until completely dissolved. Sterilize by autoclave at 121¡ÆC for 15 minutes. Cool to 45-50¡ÆC in water bath. Aseptically add 2 vials of M9 Minimal Broth supplement(MB-M0732). Mix well. Dispense in tubes.

       

       

      STORE

       

      The powder is very hygroscopic. Store the powder at room temperature, in a dry environment, in its original container tightly closed and use it before the expiry date on the label or until signs of deterioration or contamination are evident. Store prepared medium at 2 - 8¡ÆC.

       

       

       

       




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