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Middlebrook 7H 11 Broth [MB-M0981]
Medium used for the isolation and cultivation of Mycobacteria.
PACKAGE
Description |
Quantity |
Cat. No |
Middlebrook 7H 11 Broth |
500g |
MB-M0981 |
* MiddleBrook OADC Enrichment supplement (Çʼö÷°¡) |
1 vial / 50ml/ ³ÃÀå |
MB-M3021 |
* Glycerol supplement (Çʼö÷°¡) |
50ml/ ½Ç¿Â |
MB-G1821 |
* Middlebrook OADC Enrichment supplement [MB-M3021]
Á¦Ç°ÀÇ °æ¿ì Middlebrook 7H10 supplement
[MB-M1835] Á¦Ç°°ú ÅëÇյǾú½À´Ï´Ù.
* MiddleBrook OADC Enrichment supplement (º°µµ ±¸¸Å ÇÊ¿ä)
Contents |
mL, g |
1 vial contents (each vial is sufficient for 500L of
medium) |
|
Oleic Acid |
0.025 mL |
Albumin Fraction V, Bovine |
2.5 g |
Glucose |
1.0 g |
Catalase |
0.002 g |
Sodium Chloride |
0.425 g |
PROCEDURE
Suspend 5.49 G of powder in 900 mL of distilled or deionized water. Add 5
mL of Glycerol supplement (MB[1]G1821). Heat to boiling
until completely dissolved. Sterilize by autoclave at 121¡ÆC for 15 minutes.
Cool to 45 - 50¡ÆC in water bath. Aseptically add 2 vials of Middlebrook OADC
Enrichment supplement (MB-M3021). Mix well. Pour into tubes.
STORE
The powder is very hygroscopic. Store the powder at room temperature, in a
dry environment, in its original container tightly closed and use it before the
expiry date on the label or until signs of deterioration or contamination are
evident. Store prepared medium at 2-8¡ÆC.