* Mycobacterium
leprae¸¦ Á¦¿ÜÇÑ
Mycobacterium ¼ÓÀÇ ¹è¾çÀ» À§ÇÑ ¹èÁöÀÔ´Ï´Ù.
* ¹èÁö 100mL Á¦Á¶ ½Ã 200mLÀÇ
Egg-Homogenate°¡ ÷°¡µÇ¸ç (ÃÑ 300mL Á¦Á¶) º°µµÀÇ °íÇüÁ¦ ÷°¡ ¾øÀÌ °è¶õ ³ë¸¥ÀÚ ¼ººÐÀ¸·Î ¹èÁö¸¦ ±»È÷´Â ¿ø¸®ÀÔ´Ï´Ù.
* Egg-Homogenate [MB-E1810] Á¦Ç°ÀÇ À¯È¿±â°£Àº ¾à 2ÁÖ·Î ¸Å¿ì ªÀ¸¹Ç·Î º»»ç Àç°í ¹× À¯È¿±â°£, ÀÔ°íÀÏÁ¤ È®ÀÎ ÈÄ ±¸¸Å¹Ù¶ø´Ï´Ù.
2% Ogawa Medium [MB-O0982]
Medium used for the isolation and
cultivation of Mycobacterium spp.
PACKAGE
Description |
Quantity |
Cat. No |
2% Ogawa Medium |
500g |
MB-O0982 |
Egg-Homogenate (*Çʼö÷°¡) |
50ml (³ÃÀå) |
MB-E1810 |
Glycerol supplement (*Çʼö÷°¡) |
50ml (½Ç¿Â) |
MB-G1821 |
Malachite Green Solution 2% (*Çʼö÷°¡) |
1 vial / 10ml (³ÃÀå) |
MB-M0745 |
* Malachite Green Solution 2%
Contents |
g |
1 vial contents (each vial is sufficient for 500mL of
medium) |
|
Malachite Green |
0.2 g |
PROCEDURE
Suspend 2.6 G of powder in 100 mL of distilled or deionized water. Add 4
mL of Glycerol supplement (MB-G1821) and 4 mL of Malachite Green Solution 2%
(MB-M0745). Heat to boiling until completely dissolved. Sterilize by autoclave
at 121¡ÆC for 15 minutes. Cool to 45 - 50¡ÆC in water bath. Aseptically and 200
mL of Egg-Homogenate (MB-E1810). Mix well. Pour into tubes to 5 mL. Arrange
tubes in a slanted position. Coagulate and inspissate at 85 - 90¡ÆC for 60
minutes.
STORE
The powder is very hygroscopic. Store the powder at room temperature, in a
dry environment, in its original container tightly closed and use it before the
expiry date on the label or until signs of deterioration or contamination are
evident. Store prepared medium at 2 - 8¡ÆC.