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MRS Broth without Glucose [MB-M0757]
Medium used for the cultivation and enumeration of Lactobacilli.
PACKAGE
Description |
Quantity |
Cat. No |
MRS Broth without Glucose |
500g |
MB-M0757 |
PROCEDURE
Suspend 35.15 G of powder in 1L of distilled or deionized
water. Aseptically add 20.0 G of glucose. Heat to boiling until completely
dissolved. Sterilize by autoclave at 121¡ÆC for 15 minutes. Cool to 45 - 50¡ÆC in
water bath. Mix well. Pour into tubes.
STORE
The powder is very hygroscopic. Store the powder at room
temperature, in a dry environment, in its original container tightly closed and
use it before the expiry date on the label or until signs of deterioration or contamination
are evident. Store prepared medium at 2 - 8¡ÆC.