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Ziehl-Neelsen color kit [MB-80276]

Complete kit for coloring acid-alcohol resistant bacteria.

PACKAGE

PROCEDURE

Preparation and fixing

• On clean slides make a smear of the pathological material or of the culture. Leave to dry in the air and fix by passing rapidly over the flame, or by using other fixing methods. Do not overheat the sample when fixing.

Coloring

In all phases of the coloring, the preparation must be rinsed with water free of acid-alcohol resistant species.

• Cover the slide with the Fuchsin Solution. Heat the slide slowly until steam forms. Continue heating the slide for 3-5 minutes. Leave to cool and then rinse with water.

• Decolor with the Acid-Alcohol Solution for as long as the preparation releases color (about 1-2 minutes). Then rinse with water.

• Cover the slide with Methylene Blue Solution. Leave in contact for 30-60 seconds. Then rinse with water and leave to dry.

• Examine the preparation under the microscope with the objective for immersion.

STORE

Store the Ziehl-Neelsen Color Kit at room temperature in its original package. Keep away from sources of heat and avoid excessive changes of temperature. In such conditions the Ziehl-Neelsen Color Kit will remain valid until the expiry date indicated on the label. Do not use beyond that date. Eliminate without using if there are signs of deterioration (change in the color of the solutions or presence of substantial precipitates).