* Tryptophanase¸¦ »ý¼ºÇÏ´Â ¹Ì»ý¹°Àº Àε¹, ÇÇ·çºê»ê, ¾Ï¸ð´Ï¾Æ¿¡¼
TryptophanÀ» °¡¼öºÐÇØÇϸç, IndoleÀÇ Á¸Àç´Â
Kovac¡¯s reagent¸¦ »ç¿ëÇÏ¿© °¨ÁöÇÒ ¼ö ÀÖ½À´Ï´Ù.
* ÇÏ´Ü °ü·ÃÁ¦Ç° ½ÇÇèÇϱ⠹öưÀ»
Ŭ¸¯ÇÏ½Ã¸é ½ÇÇè¹æ¹ý¿¡ ´ëÇÑ ³»¿ëÀ» È®ÀÎÇÏ½Ç ¼ö ÀÖ½À´Ï´Ù.
Indole Test [MB-88017]
Rapid test for detecting indole production.
PACKAGE
Description |
Quantity |
Cat. No |
Proteic Substrate Mixture |
30 tubes |
MB-88017 |
Kovac¡¯s reagent (ŰƮ¿¡ Æ÷ÇԵǾî ÀÖÀ½) |
3 ml |
MB-9410 |
INTERPRETATION OF THE RESULTS
Color of medium |
Indole Test |
Presence of red ring |
Positive |
Absence of red ring |
Negative |
QUALITY CONTROL FOR USE
Quality control microorganism |
||
Microorganism |
ATCC |
Indole Test |
Escherichia coli |
25922 |
+ |
Krebsiella pneumonia |
13883 |
- |
PRINCIPLE OF THE METHOD
Indole test´Â Æ®¸³ÅäÆÄ³ª¾ÆÁ¦¸¦ »ý¼ºÇÏ´Â
¹Ì»ý¹°ÀÌ Àε¹, ÇÇ·çºê»ê, ¾Ï¸ð´Ï¾Æ¿¡¼ Æ®¸³ÅäÆÇÀ» °¡¼öºÐÇØÇϸç
Àε¹ÀÇ Á¸À縦 Kovac¡¯s reagent¸¦ ÅëÇØ ¾Ë ¼ö Àִ ŰƮÀÔ´Ï´Ù. ³ë¶õ»öÀÎ Kovac¡¯s reagent´Â Àε¹°ú ¹ÝÀÀÇÏ¿© ½ÃÇè°ü Ç¥¸é¿¡
ºÓÀº»öÀ» ¶ç°Ô µË´Ï´Ù.
PROCEDURE
• ŰƮ¸¦ ²¨³»¾î ½Ç¿Â¿¡ Àá½Ã ¹æÄ¡ÇÕ´Ï´Ù.
• ¸ê±ÕµÈ ·çÇÁ¸¦ »ç¿ëÇÏ¿© ¼ø¼ö ¹è¾çµÈ ÀÇ½É Áý¶ôÀ» Æ©ºê¿¡ Á¢Á¾ÇÕ´Ï´Ù.
• 36¡É¿¡¼ 1~4½Ã°£¿¡¼ ÃÖ´ë 24½Ã°£µ¿¾È ¹è¾çÇÕ´Ï´Ù.
• Kovac¡¯s reagent¸¦ 2~3¹æ¿ï Á¢Á¾ÇÑ
µÚ °á°ú¸¦ È®ÀÎÇÕ´Ï´Ù.
STORE
Store Indole Test at 2-6 ¡ÆC in its original package. In such conditions
the product is valid until the expiry date shown on the label. Do not use after
expiry date. Dispose of them if they show signs of deterioration.