[Decolorising Solution (Gram staining)]

미생물진단 Test Kit > 염색시약

Decolorising Solution (Gram staining)

상품리뷰

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* 세균의 그람 염색에 사용되는 시약 중 탈색과정에 사용되는 탈색액입니다.

* 그람염색에 필요한 4종류 시약을 모두 세트로 구매하시려면 Gram Color Kit [MB-80293] 제품을 구매하시면 됩니다.

* 관련제품 실험방법 링크를 클릭하면 그람 염색법에 관한 블로그 포스팅으로 이동합니다.

Decolorising solution (Gram staining) [MB-80295]

PACKAGE

Description	Quantity	Cat. No
Decolorising solution (Gram staining)	1000mL	MB-80295

PROCEDURE (그람염색 과정에 대한 설명입니다)

Preparation and fixing

On clean slides, make a smear of the culture or pathological material. Leave to dry in the air and fix by heat, passing rapidly over the flame. Do not overheat the sample when fixing. Other fixing methods may be used.

Staining

1. Cover the slide with the Crystal Violet Solution. Wait 1 minute, then rinse gently with water.

2. Cover the slide with the Lugol PVP Solution. Wait 1 minute.

3. Decolor with the decolorizing Solution for as long as the preparation releases color (about 30-60

seconds), then rinse delicately with water.

4. Cover the slide with the Safranine Solution. Wait 30-60 seconds, then rinse delicately with water.

5. Dry.

6. Examine the preparation under the microscope with the objective for immersion.

STORE (그람염색 키트에 대한 보관 방법입니다)

Store Gram Color Kit at 10-25°C in the original packaging. Keep away from sources of heat and avoid excessive changes of temperature. In such conditions the product Gram Color Kit will be valid until the expiry date shown on the label. Do not use beyond that date. Eliminate without using if there are signs of deterioration (changes in the color of the solutions or presence of substantial precipitates).