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Chocolate Agar [MB-C2143]
A highly nutritious medium for the isolation and
cultivation of fastidious microorganisms, especially Neisseria and Haemophilus
species.
PACKAGE
Description |
Quantity |
Cat. No |
Chocolate Agar |
500g |
MB-C2143 |
Chocolate Agar |
*500ml x 10 |
MB-C2143M |
*Sheep Blood
Defibrinated (ÅÃ1) |
50ml/³ÃÀå |
MB-S1876 |
*Horse Blood
Defibrinated (ÅÃ1) |
50ml/³ÃÀå |
MB-H1883 |
*Growth
Factor Mixture (with Hydration Fluid) (Çʼö÷°¡) |
1 vial/ 5ml/ ³Ãµ¿ |
MB-G2521 |
* 500ml x 10 Á¦Ç°ÀÇ °æ¿ì, 500ml ¹èÁö¸¦ Á¦Á¶ÇÒ ¼ö ÀÖ´Â ÆÄ¿ì´õ ¾ç¸¸Å ¼ÒºÐµÇ¾îÀÖ´Â Á¦Ç°ÀÔ´Ï´Ù.
* Growth Factor Mixture (with Hydration Fluid) (º°µµ ±¸¸Å ÇÊ¿ä)
Contents |
mg |
1 vial contents (each vial is sufficient for 500L of
medium) |
|
Adenine |
10.0 mg |
p-Aminobenzoic Acid |
0.13 mg |
Cocarboxylase |
1.0 mg |
L-Cysteine |
259.0 mg |
L-Cystine |
11.0 mg |
Ferric Nitrate |
0.2 mg |
Glucose |
1.0 g |
L-Glutamine |
100.0 mg |
Guanine |
0.2 mg |
NAD |
2.5 mg |
Thiamine |
0.03 mg |
Vitamin B12 |
0.1 mg |
PROCEDURE
Suspend 33.0 G in 920 mL of distilled or deionized water.
Heat to boiling completely dissolve the medium. Sterilize by autoclave 121 ¡ÆC
for 15 minutes. Cool to 45-50¡ÆC in water bath. Aseptically add 70 mL of Horse Blood
Defibrinated (MB-H1883) or Sheep Blood Defibrinated (MB-S1876) and heat in a
water bath at 80¡ÆC for 10 minutes. Cool to 45-50¡ÆC in water bath. Aseptically 2
vials of Growth Factor Mixture (with Hydration Fluid) (MB-G2521). Mix well.
Dispense into petri dishes.
STORE
The powder is very hygroscopic. Store the powder at room
temperature, in a dry environment, in its original container tightly closed and
use it before the expiry date on the label or until signs of deterioration or
contamination are evident. Store prepared medium at 2 - 8¡ÆC.