* Brucella spp. 의 분리 및 배양에 사용되는 배지입니다.
* Brucella Agar
Base 제품, 식품공전 규격 제품 [MB-B1079K] 이 Brucella Agar 로 통합되었습니다.
Brucella Agar [MB-B1079]
Medium used for the isolation and cultivation of Brucella spp.
* Equally use
with MFDS (MB-B1079K) and Brucella Agar Base.
* 식품공전 규격과 동일합니다.
PACKAGE
Description |
Quantity |
Cat. No |
Brucella Agar |
500g |
MB-B1079 |
Brucella Agar |
*500ml x 10 |
MB-B1079M |
* Bovine
Serum (필수) |
100ml / 냉동 |
MB-B1887 |
* Brucella
Selective supplement (선택) |
1 vial / 5ml / 냉동 |
MB-B1803 |
* 500ml x 10 제품의 경우, 500ml 배지를 제조할 수 있는 파우더 양만큼 소분되어있는 제품입니다.
* Brucella Selective supplement (필요 시 첨가 - 별도 구매 필요)
Contents |
g |
1 vial contents (each vial is sufficient for 300mL of
medium) |
|
Polymyxin B |
2,500 IU |
Bacitracin |
12,500 IU |
Cycloheximide |
0.05 g |
Nalidixic Acid |
0.0025 g |
Nystatin |
50,000 IU |
Vancomycin |
0.01 g |
PROCEDURE
Suspend 43.1 G of powder in 950 mL or 900 mL of distilled
or deionized water. Heat to boiling until completely dissolved. Sterilize by
autoclave at 121°C for 15 minutes. Cool to 45 - 50°C in water bath. Aseptically
add 5 - 10% of sterile Bovine Serum (MB-B1887, required heat inactivation at
56°C for 30 miniutes). If necessary, add 2 vials of Brucella Selective
supplement (MB-B1803). Mix well. Pour into petri dishes.
STORE
The powder is very hygroscopic. Store the powder at room
temperature, in a dry environment, in its original container tightly closed and
use it before the expiry date on the label or until signs of deterioration or
contamination are evident. Store prepared medium at 2 - 8°C.